关键词: 小鼠诺如病毒, 全基因组, 遗传进化分析

Abstract: Objective　To investigate the prevalence of murine norovirus (MNV) in commercial laboratory mice and the genomic characteristics of circulating strains.Methods　Reverse transcription polymerase chain reaction (RT-PCR) was employed to detect MNV infections in commercial Kunming mice from Beijing(10 samples), Shandong(10 samples), and Liaoning (15 samples). Positive samples were subjected to whole genome sequencing analysis.Results　The Kunming mice from different regions were infected with varying degrees of MNV. The whole genome sequencing and analysis of the positive sample MNV 2024BJ strain revealed a genome length of 7 382 nt, and the nucleotide homology with the representative MNV strains ranging from 88.9% to 89.9%. Structural analyses of the ORF1 cleavage site, glycosylation site, and non coding region indicated similarities between this strain and the representative strains of MNV genotype 1. Phylogenetic analysis demonstrated that this strain is closely related to domestic Guangzhou/K162/09/CHN and BJ10-2062 strains, and belongs to the same evolutionary branch.Conclusion　MNV infection is common in commercial Kunming mice, with certain  differences in nucleotide homology with reported strains at home and abroad, the potential risks caused by it should be further emphasized.

Key words: murine norovirus, complete genome, genetic evolution analysis